Abstract: The structural design of the new exhibition greenhouse (cold greenhouse and butterfly greenhouse) in the north area of Shanghai Botanical Garden was introduced, including design parameters and load value, foundation and column footing design and upper steel structure design. The shape coefficients of wind load on the structure surface were obtained by computational fluid dynamics numerical simulation, which were used as the design basis of the characteristic values of wind load together with the code values. The design methods of key problems in single-layer space latticed structure were provided, such as member effective length, rigid joint structure, structural elastic performance, structural stability ultimate bearing capacity and steel structure fire safety. The analysis results showed that each performance of the structure can meet the design provisions of relevant codes, which could provide reference for similar engineering design.