Abstract: With the rapid development of rail vehicles, passengers pay more attention to ride comfort while paying attention to speed and punctuality. Firstly, this paper studies the Sperling index, UIC 513 standard and ISO 2631 standard commonly used in railway system at home and abroad. Then it from the test requirements, calculation methods and evaluation criteria are compared . Finally, based on MATLAB, the ride comfort program of rail vehicles is developed, and the data collected on site were included for analysis and evaluation. This study provides a good support for the comprehensive evaluation of the ride comfort of rail vehicles and the standard adaptability problems faced by China 's rail transit equipment export abroad.