Abstract: In order to provide a theoretical basis for spore reproduction, the orthogonal experimental design was used to explore the influence of several media factors on the regeneration process of Parathelypteris glanduligera (Kunze) Ching. Following results were obtained: by 9 groups of orthogonal tests, the germination rate of the spores was between 25% and 70%, pH had a significant effect (p＜0.05) on spore germination, and no other media factors had a significant effect on spore germination.The prothallium exhibited varying degrees of proliferation, ranging from 0.04 g to 0.75 g under the test treatment. Naphthaleneacetic acid (NAA) and indolebutyric acid (IBA) had a significant effect on prothallium proliferation, and the significant values were p＜0.01 and p＜0.05, respectively, other media factors were not significant. The maximum number of sporophyte transformation was 5.33 after 30 d test culture. The transformation were successfully under media containing quartern Murashige and Skoog culture medium (MS culture medium) or half MS culture medium among the three media factors, while the sporophyte could not be induced under the medium adding MS culture medium. Therefore inorganic salts had a significant effect on sporophyte induction, and other media factors do not have signifcant effect.The sporophyte developed adventitious roots on the 10 d when cultured in rooting medium, and at 14 d of test culture, the maximum number of roots produced was 5. Agar and inorganic salts had a significant effect on rooting, and the significant values were p＜0.01 and p＜0.05, respectively.