Study on Embryogenic Callus Induction and Anti-Browning of Emmenopterys henryi Oliv.

The leaf, petiole and stem segments of Emmenopterys henryi Oliv. were used for the induction of embryogenic callus. To alleviate callus browning in subculture of callus, anti-browning agents were screened. Results showed that petioles sterilized by 75% alcohol for 60 s and 0.2% HgCl₂ for 15 min successively were optimal for embryonic callus induction. The optimum formula for embryogenic callus induction and callus browning inhibition during subculture were decided.